阿尔法4整合素介导淋巴细胞的附着和在生理流动下滚动
C Berlin1, R F Bargatze, J J Campbell
1Department of Pathology, Stanford University, California 94305.
Cell
|February 10, 1995
概括
阿尔法4 (CD49d) 整合素在剪切流下启动淋巴细胞粘附,独立于选择素. 这些整合素与β2整合素不同,对于白细胞的初始相互作用和附着是至关重要的.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 白细胞-内皮细胞相互作用对免疫反应至关重要.
- 选择素启动白细胞在生理剪切下滚动,先于整合素介导的停止.
- 贝塔2 (CD18) 整合素需要选择因参与中性粒细胞粘附.
研究的目的:
- 研究α4 (CD49d) 整合素在剪切流下启动白细胞粘附中的作用.
- 为了确定alpha 4整合素是否可以独立于selectins调节粘附.
- 阐明α4整合素介导粘附的机制,包括连接体相互作用和地形呈现.
主要方法:
- 在体外剪切流量测试以评估淋巴细胞结合和滚动.
- 临床研究的淋巴细胞附着在自己的膜静脉.
- 扫描电子显微镜可视化淋巴细胞上的整蛋白分布.
主要成果:
- 阿尔法4 (CD49d) 整合素在剪切下启动淋巴细胞结合,没有选择性参与.
- 阿尔法4整合素连接体 (MAdCAM-1,VCAM-1) 支持滚动和取决于激活的停止.
- 阿尔法4β7在体内调解L-选择因独立的淋巴细胞粘附.
- 阿尔法4β7集中在微型菌上,便于初始接触,而β2整合素被排除在外.
结论:
- 阿尔法4整合素,但不是β2整合素,可以在流动条件下启动白细胞粘附.
- 微上α4整合素的地形呈现有助于它们在启动粘附方面的作用.
- 这些发现揭示了由α4整合素介导的淋巴细胞粘附启动的独特机制.
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