对依赖IgE的基因组胺释放因子进行分子鉴定
S M MacDonald1, T Rafnar, J Langdon
1Department of Medicine, Johns Hopkins University School of Medicine, Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA.
Science (New York, N.Y.)
|August 4, 1995
概括
研究人员在过敏患者中确定了一种与免疫球蛋白E (IgE) 相关的组胺释放因子 (HRF). 这种因子HRF在慢性过敏疾病中起作用,并导致基细胞释放组胺.
科学领域:
- 免疫学 免疫学 免疫学
- 过敏研究 研究过敏
背景情况:
- 过敏性疾病涉及复杂的免疫反应.
- 免疫球蛋白E (IgE) 在过敏反应中起着核心作用.
- 胰岛素释放是过敏反应的关键调解者.
研究的目的:
- 识别和净化参与IgE依赖性过敏反应的组胺释放因子 (HRF).
- 为了研究HRF,IgE和胰岛素释放之间的关系.
- 探索HRF在慢性过敏疾病中的作用.
主要方法:
- 从过敏患者的生物液体中净化HRF.
- HRF的氨基末端测序.
- 再组合HRF的生产.
- 测试人类基细胞的复合HRF,以测试基素释放活性.
- 利用多克隆抗体中和HRF活动.
主要成果:
- 鉴定并净化了HRF来自儿童的亚托皮性淋巴细胞和过敏患者的生物液体.
- HRF显示对小鼠p21和人类p23.3的同质性.
- 再组合HRF诱导了IgE依赖的组织胺素从人类基细胞的亚群释放.
- 抗体有效地识别并消除了HRF的生物活性.
结论:
- HRF是一种依赖IgE的基因组胺释放的调解者.
- HRF与IgE异质性有关.
- HRF与慢性过敏疾病的发病有关.
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