p19Skp1和p45Skp2是环林A-CDK2S相激酶的重要组成部分
H Zhang1, R Kobayashi, K Galaktionov
1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724, USA.
Cell
|September 22, 1995
概括
研究人员确定p45对环林A-CDK2激酶活性至关重要,对于细胞周期S阶段进入至关重要. 转化细胞中的p45增加可能通过改变细胞周期控制来驱动细胞转化.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 环素A-CDK2复合体对于正常人体纤维细胞S阶段进入至关重要.
- 转变的细胞通常表现出改变的环素A-CDK2复合体组成,缺乏p21并结合p9CKS1/CKS2,p19和p45.
研究的目的:
- 研究p19和p45在环林A-CDK2复合物的形成和功能中的作用.
- 确定p45丰富度在细胞转化中的重要性.
主要方法:
- 分离了编码为p19和p45的cDNA.
- 在体外溶解试验测试以研究蛋白质相互作用.
- 在体内功能研究使用微注射抗体和抗意义寡核酸.
主要成果:
- 对于p19在体外与环林A-CDK2结合,需要p45.
- p45和p19在体外没有显著改变环林A-CDK2激酶活性.
- 在体内抑制p45功能阻断了正常细胞和转化细胞的S阶段进入.
- 在许多转化细胞类型中,p45的丰度显著增加.
结论:
- p45是S相进展所需的环林A-CDK2激酶活性的一个重要组成部分.
- 转化细胞中的p45水平升高可能导致异常的细胞周期控制和细胞转化.
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