在流下的白细胞粘附中,微细胞受体呈现的中心作用
U H von Andrian1, S R Hasslen, R D Nelson
1Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA.
Cell
|September 22, 1995
概括
白血球微增强了细胞粘附. 呈现L-选择素 (CD62L) 在微小上,而不是细胞体,对于在流条件下启动白细胞粘附至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 白细胞对内皮的粘附对于免疫反应至关重要,需要在血液流动下进行初始接触的特定机制.
- 一个关键的粘附分子L-selectin (CD62L) 通常被发现聚集在白细胞微小细胞上,这表明它在粘附启动中发挥了作用.
研究的目的:
- 研究L-选择素在白细胞粘附启动中的微状呈现与平面细胞体呈现的关键作用.
- 确定粘附分子的空间定位是否影响它们在流动下细胞表面相互作用中的功能.
主要方法:
- 淋巴细胞感染了L-选择素和CD44的仿制结构,这种粘附分子通常不属于微型菌.
- 在静态和流动条件下,对野生类型和仿真变质剂结合的抗体 (MAb) 的分析.
- 评估L-选择素的莱克活性及其对与原生配体接触的功能后果.
主要成果:
- 化学L-selectin结构表明CD44域将L-selectin直接导向细胞体,而L-selectin域则促进CD44在微型病毒上集群.
- 微细菌呈现的L-选择素在流动条件下显著增强了抗体结合和初始细胞接触 (结合),而不是在平面细胞体上的呈现.
- 观察到等效的莱克活性,但功能粘附启动被微型 localization 显著改善.
结论:
- 受体拓,特别是白细胞微小的呈现,在初始白细胞粘附的效率中起着至关重要的作用.
- 这种空间布局代表了一种控制白细胞贩运和免疫细胞迁移的新型调节机制.
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