结合性红色体BFU-E和CFU-E原始体的产生不需要红色素或红色素受体
H Wu1, X Liu, R Jaenisch
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Cell
|October 6, 1995
概括
红色素 (EPO) 和其受体 (EPO-R) 中的零突变导致由于最终的红色素生成失败而导致胚胎致死性. EPO和EPO-R对于胎儿红细胞的产生和生存至关重要.
科学领域:
- 血液学 血液学 血液学
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 红色素 (EPO) 是红细胞生产的关键调节剂.
- 环氧化受体 (EPO-R) 调解了EPO的生物效应.
- 了解EPO和EPO-R在红色素形成中的作用至关重要.
研究的目的:
- 为了研究EPO和EPO-R在红细胞生成过程中的体内功能.
- 为了确定替代带或受体是否可以补偿EPO/EPO-R损失.
主要方法:
- 在Epo和EpoR基因中产生具有零突变的小鼠.
- 在异性和同性突变动物中对红色素形成的分析.
- 在胚胎发育过程中评估原始和最终的红质形成.
主要成果:
- 同卵性Epo和EpoR无基因突变导致相同的表型,包括13日左右的胚胎致死性.
- 突变胚胎显示原始红色体育的减少和最终的胎儿肝脏红色体育失败.
- 红状腺原始体 (BFU-E,CFU-E) 存在,表明EPO/EPO-R不需要用于血统承诺或早期原始体增殖/分化.
- 在体内,EPO和EPO-R对于CFU-E的扩散,生存和终端分化至关重要.
结论:
- 埃博和EPO-R对于 in vivo 确定的红色素形成是不可或缺的.
- 没有观察到其他配体或受体的补偿机制.
- 对于红状腺发育的晚期阶段,特别是CFU-E成熟和生存,EPO信号传递至关重要.
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