由瘤转录因子IRF-2激活细胞循环调节的基因组基因
P S Vaughan1, F Aziz, A J van Wijnen
1Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA.
Nature
|September 28, 1995
概括
组织素H4基因FO108受组织素核因子M (HiNF-M) 的调节,被确定为干扰素调节因子2 (IRF-2). 这种蛋白质将细胞循环的进展与DNA复制联系在一起,影响细胞生长.
科学领域:
- 分子生物学分子生物学
- 细胞循环规则 细胞循环规则
- 基因表达 基因表达
背景情况:
- 人类基因组素H4基因FO108表现出细胞周期依赖的转录,在早期S阶段达到峰值.
- 激活H4基因基因需要细胞循环元素 (CCE),该元素与特定的蛋白质因子,基因核因子M (HiNF-M) 结合.
研究的目的:
- 为了净化HiNF-M并确定其分子身份.
- 阐明HiNF-M在调节组素H4基因转录中的作用及其与细胞循环进展的联系.
主要方法:
- 电泳运动转移试验 (EMSA) 用于研究蛋白质-DNA相互作用.
- 进行了HiNF-M的净化.
- 用复合干扰素调节因子2 (IRF-2) 和IRF-1来测试它们与CCE的结合及其对转录的影响.
主要成果:
- 净化了HiNF-M并确定为干扰素调节因子2 (IRF-2),这是已知的干扰素反应的负调节因子.
- 再组合IRF-2和IRF-1特别与CCE结合.
- IRF-2和IRF-1激活了H4基因组蛋白基因的转录.
结论:
- 干扰素调节因子2 (IRF-2) 是一种蛋白质因子,它与细胞循环元素 (CCE) 结合,并激活 histone H4 基因转录.
- 在G1/S阶段过渡时,IRF-2在合DNA复制和细胞周期进展方面发挥着功能性作用.
- 这些发现将因其致癌潜力而闻名的IRF-2与参与细胞增殖的关键基因联系起来.
相关概念视频
NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...


