将TAFs与核心元素结合,可以通过RNA聚合酶II指导促进子选择性
C P Verrijzer1, J L Chen, K Yokomori
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California at Berkeley 94720-3204, USA.
Cell
|June 30, 1995
概括
转录因子TFIID,包括TAF (TBP相关因子),对于基底转录至关重要. 特定的TAF组合通过与核心促销元件相互作用来调节促销器识别和转录效率.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 生物化学 生物化学
背景情况:
- 核心促进体识别和基底转录效率机制尚未完全理解.
- TAFs (TBP相关因子) 和TFIID复合体在基底转录中的作用需要进一步研究.
研究的目的:
- 评估TAFs和TFIID复合体在指导基底促进器功能的潜在作用.
- 确定有效利用促进剂的最小TAF要求.
主要方法:
- 利用复制的转录反应来分析TFIID和TBP的功能.
- 将不同的部分TBP-TAF组件进行比较,以确定基本组件.
主要成果:
- 与单独的TBP不同,TFIID可以区分不同的核心发起人.
- 对于高效的启动器和下游元件利用,三元体TBP-TAFII250-TAFII150复合体是最低要求的.
- 根据促进体结构,TAFs调节了TFIID-促进体相互作用的稳定性.
结论:
- 在基底转录中,TAF对于促进体选择性至关重要.
- TAFs通过与核心促进元件的直接相互作用来调节转录效率.
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