14-3-3蛋白的泽塔异型的晶体结构是14-3-3蛋白
D Liu1, J Bienkowska, C Petosa
1Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Nature
|July 13, 1995
概括
14-3-3蛋白家族调节细胞内信号传递. 研究人员确定了主要的14-3-3蛋白质的晶体结构,揭示了各种细胞相互作用的保留结合槽.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 14-3-3蛋白质是细胞内信号通路的关键调节者.
- 它们与瘤基因和原型瘤基因产物相互作用,影响细胞过程.
- 之前的研究表明它们在激酶激活和诱导细胞成熟方面的作用.
研究的目的:
- 为了确定主要哺乳动物14-3-3蛋白质异型体的晶体结构.
- 阐明14-3-3蛋白与其他细胞分子相互作用的结构基础.
主要方法:
- 使用X射线晶体学来确定蛋白质结构.
- 取得的分辨率为2.9安格斯特罗姆.
主要成果:
- 晶体结构揭示了一个二面蛋白质,由每个子单位的九个反平行螺旋组成.
- 确定了一个能够绑定额外螺旋的中央两槽.
- 在各个物种中观察到二聚体接口和连接体结合表面的保留残留物.
结论:
- 鉴定出来的沟表明了14-3-3蛋白与多种细胞合作伙伴相互作用的一般机制.
- 在脊椎动物,酵母和植物中表明14-3-3蛋白的结构和功能保护.
更多相关视频
相关概念视频
Protein Organization
Proteins are one of the fundamental building blocks of life that carry out many diverse functions in the cell. Proteins are assembled from amino acids. The sequence of amino acids is known as the primary structure of a protein. Local interactions of individual amino acids cause the linear chain to fold into the secondary structures. Interactions of distant amino acids lead to further folding of the protein—the tertiary structure. The assembly of multiple folded chains (subunits) is known as...
Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation which is critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.Protein Structure Is Critical to Its Biological FunctionProteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.A protein's shape is critical to its function. For example, an enzyme can...
Protein Organization
Proteins are one of the fundamental building blocks of life that carry out many diverse functions in the cell. Proteins are assembled from amino acids. The sequence of amino acids is known as the primary structure of a protein. Local interactions of individual amino acids cause the linear chain to fold into the secondary structures. Interactions of distant amino acids lead to further folding of the protein—the tertiary structure. The assembly of multiple folded chains (subunits) is known as...
Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.
Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...


