在缺乏表皮生长因子受体的小鼠中表皮不成熟和多器官衰竭
P J Miettinen1, J E Berger, J Meneses
1Department of Growth and Development, University of California, San Francisco 94143-0640, USA.
Nature
|July 27, 1995
概括
缺少表皮生长因子受体 (EGF-R) 基因的小鼠表皮细胞发育受损,并没有在8天后生存. 这凸显了EGF-R在哺乳动物胚胎发育中的关键作用.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 表皮生长因子受体 (EGF-R) 是一种原型的氨酸激酶受体.
- 通过像EGF和TGF-alpha这样的配体激活EGF-R诱导细胞增殖和分化.
- EGF-R在哺乳动物中得到广泛表达,对胚胎发育至关重要,尽管在造血细胞中不存在.
研究的目的:
- 调查EGF-R及其配体的发育和生理作用.
- 为了了解EGF-R基因无活化 in vivo的后果.
主要方法:
- 基因向制造EGF-R缺乏 (EGF-R-/-) 的小鼠.
- 对EGF-R-/-小鼠进行表型分析.
主要成果:
- EGF-R-/-小鼠在出生后存活了长达8天.
- 在多个器官中观察到表皮发育障碍,包括皮肤,肺和胃肠道.
- 这些发现表明,EGF-R在器官生成中起着至关重要的作用.
结论:
- 在正常的哺乳动物胚胎发育中,EGF-R信号是不可或缺的.
- 破坏EGF-R功能会导致严重的发育缺陷和致命性.
- 这项研究强调了EGF-R途径在上皮组织形成中的重要性.
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