MSX1 抑制了纤维细胞 × 10T1/2 细胞杂交体中的 myoD 表达
1Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA.
Cell
|August 25, 1995
概括
人类染色体4通过家庭盒基因MSX1.1.抑制myoD基因激活. MSX1直接抑制myoD转录,揭示了一个新的基因调节层.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- myoD基因对于肌肉细胞的分化至关重要.
- 转移人类染色体11激活了10T1/2细胞中的myoD.
- 额外的人类染色体可以抑制这种激活.
研究的目的:
- 为了确定负责抑制myoD激活的因素.
- 为了阐明myoD转录抑制的机制.
- 确定 homeobox 基因在 myoD 调节中的作用.
主要方法:
- 染色体转移和混合细胞的形成.
- 记者基因测试以评估转录活性.
- MSX1基因表达分析和操纵 (强制表达,反意义).
- 染色体免疫沉检测蛋白质-DNA结合.
主要成果:
- 人类染色体4在转录水平上抑制myoD激活.
- 抑制活性映射到含有MSX1基因的4p染色体.
- MSX1在myoD被抑制的细胞中表达,并直接抑制myoD增强剂活性.
- 反义MSX1可以逆转由染色体4调解的抑制.
结论:
- MSX1充当了myoD基因的转录抑制剂.
- MyoD是homeobox基因调节的直接目标.
- 这项研究揭示了一种涉及MSX1在肌肉发育中的新型调节途径.
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