失败的犬类和人类心脏细胞对β2上腺素刺激的反应
R A Altschuld1, R C Starling, R L Hamlin
1Department of Medical Biochemistry, Ohio State University, Columbus 43210-1218, USA.
Circulation
|September 15, 1995
概括
心力衰竭增加了狗心脏细胞对β2上腺素刺激的敏感性. 失败的人类心脏细胞也具有功能性β2-上腺素受体,这表明在心脏病中发挥了作用.
科学领域:
- 心脏病学 心脏病学
- 分子药理学分子药理学
- 细胞生理学 细胞生理学
背景情况:
- 失败的人类心脏表现出降低的β1-上腺素受体,但保留β2-上腺素受体.
- 带有高血压诱导失效的狗心脏显示出升高的β2-β1-上腺素受体比率.
研究的目的:
- 为了研究心力衰竭是否会增加对隔离的犬室内心肌细胞β2上腺激素刺激的敏感性.
- 为了确认功能性β2上腺体受体在失败的人类心室的肌细胞中的存在.
主要方法:
- 从健康和衰竭的狗心脏和人类移植接受者中分离出来的心肌细胞被使用.
- 细胞被用选择性β2-上腺体激动剂辛醇刺激,并测量了细胞内 ([Ca2+]i) 过渡物.
- 在犬类肌细胞中,L型Ca2+电流和cAMP产生也被评估.
主要成果:
- 在所有研究组中,辛特罗尔显著增加了[Ca2+]i过渡幅度.
- 与正常肌细胞相比,失败的犬类肌细胞对β2上腺素刺激的反应性增加.
- β2上腺刺激增加了L型Ca2+电流,但没有提高cAMP水平或斯福兰班酸化.
结论:
- 功能性β2-上腺素受体存在于失败的人类室内心肌细胞中.
- 狗腹腔肌细胞具有功能性β2-上腺素受体,在高血压阻断诱导的失败中具有增强的反应.
- 在这个模型中,β2-上腺激素刺激的积极内效应并非由cAMP或索兰班酸化介导.
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