作为潜在的人类瘤基因的CDC25酸酶
K Galaktionov1, A K Lee, J Eckstein
1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724, USA.
Science (New York, N.Y.)
|September 15, 1995
概括
CDC25酸酶可以激活循环素依赖性激酶 (CDKs). 人类乳腺癌中CDC25B过度表达表明CDC25酸酶有助于人类癌症的发展.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 循环素依赖激酶 (CDK) 调节细胞循环.
- CDC25酸酶通过去除抑制酸盐来激活CDKs.
- 人类CDC25基因家族包括CDC25A,CDC25B和CDC25C.
研究的目的:
- 调查CDC25酸酶在瘤转化中的作用.
- 为了确定CDC25酸酶是否有助于人类癌症的发展.
主要方法:
- 使用人类CDC25A,CDC25B或CDC25C评估动物细胞中瘤焦点的形成.
- 引入致癌的Ha-RASG12V或RB1损失.
- 在小鼠中分析瘤特征.
- 在人类乳腺癌样本中量化CDC25B表达.
主要成果:
- 人类的CDC25A和CDC25B,但不是CDC25C,与Ha-RASG12V或RB1损失合作,在动物细胞中形成致癌焦点.
- 转变的细胞表现出无积体,软生长,并在裸体小鼠中形成了高等级的瘤.
- 在32%的人类原发性乳腺癌中,CDC25B过度表达.
结论:
- CDC25A和CDC25B酸酶可以促进瘤性转化.
- 乳腺癌中CDC25B过度表达表明它在人类瘤发生中起作用.
- CDC25酸酶是人类癌症发展的潜在贡献者.
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