分子克隆,功能表达和C-C化学因子受体的信号特征
K Neote1, D DiGregorio, J Y Mak
1Department of Immunology, Genentech, Incorporated, South San Francisco, California 94080.
Cell
|February 12, 1993
概括
研究人员确定了C-C化学因子受体1 (C-C CKR-1),一种与C-C化学因子结合的蛋白质. 这种受体在免疫反应中起作用,可能参与病毒免疫.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- C-C 化基因是免疫细胞贩运的关键调节者.
- 这些蛋白质会吸引淋巴细胞,单细胞,乙酸蛋白和基酸蛋白,从而影响免疫反应.
研究的目的:
- 为了识别和描述C-C化学因子的新型受体.
- 调查C-C化学酶与它们的受体的结合亲和力和信号传导能力.
- 探索C-C化学因在病毒免疫中的潜在作用.
主要方法:
- 隔离了一个编码七个跨膜跨度受体的cDNA,C-C CKR-1.
- 评估各种C-C化学基因 (MIP-1α,MCP-1,RANTES,MIP-1β) 与C-C CKR-1的结合情况.
- 测量细胞内流量以评估受体信号.
- 研究C-C化学基因与细胞巨乳病毒编码的C-C CKR-1相关基因产物的相互作用.
主要成果:
- 一个新的受体,C-C CKR-1,被确定和特征.
- C-C CKR-1 结合了人类和小鼠巨细胞炎症蛋白1α (MIP-1α),人类单细胞化疗蛋白1 (MCP-1),以及具有不同亲和力的RANTES.
- 化学基因结合亲和力与信号传导效率无关,如细胞内流量所示.
- 发现C-C化学激素与细胞巨乳病毒编码的C-C CKR-1相关蛋白质结合.
结论:
- C-C CKR-1 是多个C-C化学因子的功能性受体.
- 不同的信号反应表明了复杂的调节机制.
- 通过与病毒基因产物相互作用,C-C化学物质可能在病毒免疫力中发挥作用.
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