反转录酶活性与酵母线粒体中的成熟酶编码组II内子相关
J C Kennell1, J V Moran, P S Perlman
1Department of Molecular Genetics, Ohio State University, Columbus 43210-1292.
Cell
|April 9, 1993
概括
酵母线粒体II组内基子al1和al2编码逆转录酶. 这些酶促进RNA剪接,并可能驱动内核流动性,使用内核RNA或前mRNA作为模板.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 酵母线粒体的线粒体
背景情况:
- 第二组内子是各种生物体中发现的移动遗传元素.
- 酵母菌中的线粒体DNA (mtDNA) 含有特定的内子,包括COX1基因中的al1和al2.
- 这些内子编码具有类似逆转录酶活性的蛋白质,对于RNA剪接至关重要.
研究的目的:
- 研究与酵母线粒体II组内基子al1和al2相关的逆转录酶活性.
- 为了确定该逆转录酶的模板特异性和启动位点.
- 阐明这些内子及其编码酶在RNA剪接和潜在的反元素活性中的作用.
主要方法:
- 来自酵母线粒体的核糖蛋白颗粒的分析.
- 使用特定的内核RNA和前mRNA模板对逆转录酶活性进行表征.
- 使用突变酵母菌株,增强逆转录酶活性.
主要成果:
- 酵母线粒体含有与内子al1和al2相关的逆转录酶活性.
- 这种活动对COX1基因的内子和侧面外子具有很高的特异性.
- 反转录酶使用切除的内核RNA或COX1前mRNA作为模板,在特定地点启动cDNA合成.
结论:
- 第二组内基子al1和al2在酵母线粒体内作为反元素起作用.
- 编码的逆转录酶适用于RNA剪接,表明在遗传过程中具有双重作用.
- 这些发现提供了对内子-外子相互作用和线粒体中移动遗传元素进化的洞察.
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