在内红细胞疟疾寄生虫的营养透通道
S A Desai1, D J Krogstad, E W McCleskey
1Department of Cell Biology, Washington University, St Louis, Missouri 63110.
Nature
|April 15, 1993
概括
研究人员在疟疾寄生虫Plasmodium falciparum中确定了一个关键通道,促进了通过其膜的营养物质运输. 这一发现提供了对寄生虫生长和潜在治疗点的见解.
科学领域:
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
- 生物物理学的生物物理.
背景情况:
- 疟疾寄生虫Plasmodium falciparum在红细胞中经历了快速的无性繁殖.
- 寄生虫的生长需要大量的营养吸收,这是由于宿主细胞的低透性而具有挑战性的.
- 之前关于营养物质运输的研究仅限于孤立的寄生虫,阻碍了对单个膜运输的分析.
研究的目的:
- 通过将疟疾寄生虫与宿主细胞分离的膜研究营养物质运输机制.
- 描述寄生虫的真空膜 (PVM) 的运输特性.
主要方法:
- 在红细胞内的完好无损的Plasmodium falciparum上使用了细胞附着补丁技术.
- 在移除宿主红细胞膜后,形成的gigaohm在寄生虫上封闭.
- 在PVM中研究离子和营养物质的运输.
主要成果:
- 在PVM上确定了一个大导电通道 (140 pS),对阳离子和离子均具有透性.
- 该PVM通道非常丰富,在休息潜力时>98%的时间保持开放.
- 该通道对氨酸和葡萄糖具有透性,这表明它可以运输氨基酸和单糖.
结论:
- 已识别的PVM通道在促进Plasmodium falciparum吸收营养方面发挥着重要作用.
- 这条通道对于满足寄生虫在红细胞内循环期间的高代谢需求至关重要.
- 了解这种传输机制可能会揭示疟疾治疗的新策略.
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