细胞外接入Na,K:类似于离子通道的通道
D C Gadsby1, R F Rakowski, P De Weer
1Marine Biological Laboratory, Woods Hole, MA 02543.
概括
- 的 .
科学领域:
- 生物物理学的生物物理.
- 分子生物学分子生物学
背景情况:
- Na,K-ATPase (-) 酶对于维持细胞离子梯度至关重要.
- 它在每个循环中输送出3个离子和2个离子.
- 离子运输过程中的电荷运动与形状变化有关.
研究的目的:
- 为了研究Na,K-ATPase在离子转位过程中电荷运动的性质.
- 为了确定离子释放和重新结合的电压依赖.
- 了解和离子通道之间的功能类比.
主要方法:
- 使用电压紧,内部透析的鱼巨型轴突.
- 在缺乏的情况下,通过Na-Na交换测量单向的-22流量.
- 专注于的转位路径.
主要成果:
- Na,K-ATPase活性,虽然是电中性,但表现出电压敏感性.
- 越来越多的负膜潜能加速了Na-Na交换的速度.
- 汇率遵循了一个充满电压的Sigmoid曲线.
结论:
- 外部离子释放和重新结合是主要的电荷移动步骤.
- 细胞外离子通过内的高场通道进入结合点.
- 一部分Na,K-ATPase分子的功能类似于离子通道.
相关概念视频
Primary Active Transport
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they...
Ion Channels
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Primary Active Transport
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would not...
Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
The Role of Ion Channels in Neuronal Computation
A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.


