重组纤维细胞生长因子-1 在体内促进动脉内密性增生和血管生成
E G Nabel1, Z Y Yang, G Plautz
1Department of Internal Medicine, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109-0650.
Nature
|April 29, 1993
概括
酸性纤维细胞生长因子 (FGF-1) 基因转移到动脉中会导致内脏变厚和新的血管生长. 这表明FGF-1的存在.
科学领域:
- 血管生物学 血管生物学
- 基因治疗 基因治疗
- 心血管研究研究心血管研究
背景情况:
- 纤维细胞生长因子 (FGF),包括FGF-1和FGF-2,促进血管细胞生长和血管生成.
- 在血管病理中FGF原型的体内作用仍然不清楚.
研究的目的:
- 通过体质转基因模型研究动脉壁中FGF-1的体内功能.
- 为了确定FGF-1基因转移对猪动脉的影响.
主要方法:
- 编码FGF-1分泌形式的真核体表达载体的直接基因转移到猪动脉中.
- 对照动脉用大肠杆菌β-银酸酶基因进行了转导.
- 在基因转移后21天内密厚和新血管化的分析.
主要成果:
- FGF-1基因转移诱导了猪动脉中显著的亲密加厚.
- 在加厚的内脏中观察到新毛囊形成.
- 对照动脉没有显著变化.
结论:
- 在活体中,FGF-1会诱导动脉壁的内脏增生.
- FGF-1刺激新内膜内的血管生成,可能有助于动脉样硬化斑块的新血管化.
- 转移FGF-1基因可能为改善血液流向缺血组织提供治疗策略.
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