成年老鼠丸中丸决定基因Sry的圆形转录
1Laboratory of Eukaryotic Molecular Genetics, Medical Research Council, National Institute for Medical Research, London, England.
Cell
|June 4, 1993
概括
对于确定性别至关重要的Sry基因在小鼠丸中形成圆形RNA转录. 这些丰富的,圆形的Sry RNAs存在于细胞质中,可能是基因的结果.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
背景情况:
- 在哺乳动物中,Sry基因对于男性的性别决定至关重要.
- 成年人丸中的Sry表达率高于生殖器脊,这是其主要作用部位.
- 在成年丸中Sry的确切功能仍未确定.
研究的目的:
- 为了研究在小鼠丸中发现的Sry转录的不寻常结构.
- 描述这些Sry RNA分子的性质和局部.
- 探索这些异常转录的潜在起源.
主要方法:
- 互补DNA (cDNA) 隔离和cDNA末端的5'快速放大 (RACE).
- RNAase保护测定和逆转录聚合酶连锁反应 (RT-PCR).
- 氧核酸杂化和RNAase H消化.
主要成果:
- 丸中的Sry转录表现出一个不寻常的结构,在5'位置有3'序列.
- 这些不寻常的转录是成年老鼠丸中最丰富的Sry RNA分子.
- RNAase H消化和杂交证实了这些Sry RNA分子是圆形的.
- 在不同的老鼠亚种 (Mus musculus musculus,Mus musculus domesticus和Mus spretus) 中检测到类似的圆形Sry转录.
- 圆形RNA位于细胞质中,与多体体没有显著的关联.
结论:
- 在小鼠丸中占主导地位的Sry转录是圆形的RNA分子.
- 圆形Sry RNA的形成可能来自正常的拼接过程,受Sry位点周围独特的基因组结构的影响.
- 在细胞质中存在圆形的SryRNA表明了在成年丸中对转录后调节或功能的潜在,但尚未确定的作用.
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