从大量随机序列中分离出新的 ribozymes [参见评论]
1Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
概括
科学家们发现了新的催化RNA (核糖酶),可以有效地连接RNA分子. 这些在体外进化的 ribozymes 的结合速率是未催化反应的数百万倍.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 在RNA催化过程中.
背景情况:
- 从随机序列中使用代体内选择分离新型催化RNA (核糖酶).
- 通过对5'-三酸盐的3'-基攻击来催化RNA结合的 ribozymes 的表征,模仿蛋白质酶RNA合成.
- 非催化反应也形成了一个3',5'-二键.
相关概念视频
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...


