在脊椎动物四肢发育过程中,信号分子WNT7a和SHH之间的相互作用:背部信号调节前后背部模式
1Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Cell
|March 24, 1995
概括
背部外皮和Wnt7a信号传递对于保持Sonic hedgehog (SHH) 表达至关重要,这对于脊椎动物四肢发育和后骨模式至关重要. 这项研究揭示了dorsoventral模式信号和四肢增长之间的联系.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 脊椎动物四肢的发育涉及到顶端外皮脊 (AER),后端介质和非脊脊外皮之间的复杂信号传输.
- 来自AER的纤维细胞生长因子4 (FGF4) 和来自后半细胞体的Sonic hedgehog (SHH) 是关键的信号分子.
研究的目的:
- 调查背部外皮在四肢格局中的作用.
- 阐明信号通路,将背中中心图案与四肢外生长和前后背图案联系起来.
主要方法:
- 在成长中的四肢中实验性去除背部外皮.
- 评估Sonic Hedgehog (Shh) 的表达水平.
- 对骨元素形成的分析.
- 使用外源性SHH进行救援实验.
- 研究Wnt7a的表达和功能.
主要成果:
- 背部外皮是必要的,与FGF4一起,以保持Shh表达.
- 移除背部外皮会导致后部骨元素的损失.
- 外源性SHH可以挽救后部骨元素的损失.
- 在背部外皮表达的Wnt7a提供了Shh表达和后部结构形成所需的信号.
结论:
- 来自背部外皮的Wnt7a信号传递对于维持SHH表达和适当的四肢模式至关重要.
- 这项研究表明,肢体发育过程中,背中轴 (Wnt7a) 和前后轴 (SHH) 之间存在复杂的联系.
- 肢体的生长和模式是由WNT7a,FGF4和SHH信号通路在多个轴上的相互作用协调的.
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