FIP1基因编码了酵母前mRNA多化因子的一个组成部分,该因子直接与多A) 聚合酶相互作用
P J Preker1, J Lingner, L Minvielle-Sebastia
1Department of Cell Biology, University of Basel, Switzerland.
Cell
|May 5, 1995
概括
研究人员发现了FIP1,该基因对多基化至关重要. 这种蛋白与多A) 聚合酶 (PAP1) 相互作用,对于维持mRNA稳定性和酵母中适当的3'-end处理至关重要.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 处理RNA处理RNA处理
背景情况:
- 多基化是真核mRNA处理中的关键步骤.
- 聚甲基聚合酶 (PAP1) 是负责添加聚甲基尾巴的酶.
- 调节PAP1活性和基质特异性的因素尚未完全理解.
研究的目的:
- 为了识别和描述参与酵母mRNA多化中的新型因素.
- 为了阐明多化机械的相互作用网络.
- 了解FIP1在多基化过程中的作用.
主要方法:
- 两种混合测试以确定蛋白质相互作用.
- 在实验室中用重组蛋白质进行复合形成测试.
- 对热敏FIP1等位基因的分析.
- 使用酵母提取物进行体外3'-end加工试验.
- 共同免疫沉实验.
主要成果:
- 一个重要的基因FIP1编码了一种与酵母PAP1相互作用的蛋白质.
- FIP1与RNA14直接相互作用,RNA14是分裂因子I (CF I) 的一个子单元.
- 在FIP1的突变导致缩短的多A) 尾和减少的转录水平.
- 突变FIP1的提取物显示出多化缺陷,但没有裂变.
- 多基化活性通过多基化因子I (PF I) 恢复.
结论:
- FIP1是酵母多化机械的重要组成部分.
- FIP1充当桥梁,通过RNA14将PAP1连接到CFI.
- 这种相互作用可能会为PAP1赋予前mRNA的基质特异性.
- FIP1在调节mRNA处理和稳定性方面发挥着关键作用.
相关概念视频
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Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:35Transcription Elongation Factors
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02:41Chromatin Structure Regulates pre-mRNA Processing
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02:35Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
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01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...