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通过卵巢RNA结合蛋白bruno对oskar mRNA的翻译调节至关重要
J Kim-Ha1, K Kerr, P M Macdonald
1Department of Biological Sciences, Stanford University, California 94305-5020, USA.
Cell
|May 5, 1995
概括
在局部化之前,Oskar (osk) mRNA的翻译被抑制,以确保Drosophila的正确后体模式. 这种转化抑制,由布鲁诺蛋白与BRE结合的介导,防止过早的 osk 蛋白积累.
科学领域:
- 发育生物学是发展生物学.
- 分子遗传学 分子遗传学
- 细胞生物学 细胞生物学
背景情况:
- 奥斯卡 (osk) 蛋白质对于Drosophila的后体模式至关重要,指导纳米 (nos) 形态.
- 奥斯克mRNA定位到卵细胞的后极对于精确的奥斯克蛋白活性至关重要.
- 过早的 osk 蛋白质积累可能导致错误的身体模式.
研究的目的:
- 研究Drosophila oogenesis期间 osk mRNA的翻译控制机制.
- 在其局部化之前识别抑制 osk mRNA 翻译的因素.
- 了解转化抑制如何确保 osk 蛋白的适当的空间和时间激活.
主要方法:
- 在 oogenesis 期间 osk mRNA 局部化和翻译的分析.
- 在 osk mRNA 3'UTR.中识别调控元素 (布鲁诺反应元素 - BREs).
- 使用异质mRNA与BRE测试转化抑制的功能性测试.
主要成果:
- 奥斯克mRNA翻译在被定位到后极之前被积极抑制.
- 卵巢蛋白bruno通过结合 osk mRNA 3'UTR.中的 BREs 来调节这种翻译抑制.
- 没有转化抑制的结果是在整个卵细胞中积累 osk 蛋白质,扰乱身体的模式.
- 将BRE纳入不同的mRNA会导致卵巢翻译抑制.
结论:
- 奥斯克mRNA的转化抑制是建立Drosophila后体模式的关键调节机制.
- 布鲁诺蛋白和BRE是这个调节系统的关键组成部分,确保OSK活动仅限于后极.
- 这种机制阻止了宫外骨蛋白的产生,保持了发育的忠实性.
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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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