在室腔肌细胞中因异二醇诱导的早期和延迟脱极化后,过渡物的明显模式
G M De Ferrari1, M C Viola, E D'Amato
1CMBM, Centro Materiali e Biofisica Medica, Povo, Trento, Italy.
Circulation
|May 15, 1995
概括
早期脱极化后 (EADs) 导致心脏细胞中同步的细胞内增加,与延迟脱极化后 (DADs) 不同. 这项研究阐明了EAD和DAD期间不同的动态.
科学领域:
- 心脏病学 心脏病学
- 分子生物学分子生物学
- 身体生理学 身体生理学
背景情况:
- 早期脱极化后 (EADs) 和细胞内 ([Ca2+]i) 动态之间的关系仍然不完全理解.
- 研究这些动态对于理解心律失常至关重要.
研究的目的:
- 为了比较细胞内 ([Ca2+]i) 在早期脱极化后 (EADs) 和延迟脱极化后 (DADs) 变化的时空空间特征.
- 阐明在不同类型的心脏脱极化后处理的不同机制.
主要方法:
- 隔离的几内亚猪心室肌细胞被贴片紧并装载一个指示器 (fluo-3 AM).
- 细胞以不同的频率 (0.12 Hz) 节奏,并用异二醇 (100 nmol/L) 治疗,以诱导EAD和DAD.
- 使用光显微镜监测[Ca2+]i的时空变化.
主要成果:
- 异二醇诱导了EAD和DAD,影响了动力潜力的不同阶段.
- 在EADs期间,[Ca2+]i的增加在整个细胞中是同步的,而DADs则启动局部波.
- 在EAD期间,过渡物达到更高的峰值水平,并且与DAD相比,空间异质性较小.
结论:
- 早期脱极化后 (EADs) 与同步的,广泛的细胞内 ([Ca2+]i) 的增加有关.
- 延迟后去极化 (DADs) 显示短暂物具有局部起源和波形传播.
- 这些发现凸显了EAD和DAD之间处理机制的显著差异.
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