ORC和Cdc6p相互作用,并确定基因组中DNA复制的启动频率
C Liang1, M Weinreich, B Stillman
1Cold Spring Harbor Laboratory, New York 11724, USA.
Cell
|June 2, 1995
概括
原始识别复合体 (ORC) 和Cdc6蛋白合作,在酵母中启动DNA复制. 在ORC中的突变减少了活跃复制起源的数量,突出显示了它们的基本作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 原始识别复合体 (ORC) 对于启动真核细胞中DNA复制是必不可少的.
- 了解ORC及其相互作用蛋白在复制起源选择中的精确作用至关重要.
研究的目的:
- 调查起源识别复合体 (ORC) 在酵母中DNA复制启动中的功能.
- 识别和描述与ORC相互作用以调节复制的蛋白质.
- 阐明ORC和Cdc6p确定复制频率的机制.
主要方法:
- 在酵母 (Saccharomyces cerevisiae) 中利用了对温度敏感的orc突变物.
- 采用二维 (2D) 凝电泳来分析DNA复制的开始.
- 进行了基因选,以确定orc突变的抑制剂.
- 进行生物化学分析以确认蛋白质与蛋白质之间的相互作用.
主要成果:
- orc2-1和orc5-1突变体显示出减少了活性DNA复制起源的数量.
- 在限制性温度下,活跃的复制者数量进一步下降.
- 鉴定出CDC6是一种orc5-1突变的多复制抑制剂.
- 结果显示,Cdc6p与ORC.有功能性和物理性的相互作用.
结论:
- 在复制起源时,ORC和Cdc6p形成一个预复制复合体.
- ORC和Cdc6p合作调节全基因组范围内的DNA复制启动频率.
- 这些发现为DNA复制控制的基本机制提供了洞察力.
相关概念视频
02:31Replication in Eukaryotes
Overview
02:31Replication in Eukaryotes
Overview
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
01:29Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
01:29Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...