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神经递质释放中的突触囊泡的不同池
V A Pieribone1, O Shupliakov, L Brodin
1Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021, USA.
Nature
|June 8, 1995
概括
突触素通过将突触囊组织成不同的池来调节神经递质释放. 与突触素相关的远端池对于维持高频神经传输至关重要.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 神经终端表现出高囊释放率,是一种独特的分泌功能.
- 神经元特异性蛋白质,如突触素,参与调节神经递质释放.
- 突触素被认为通过酸化调节突触囊泡和细胞骨相互作用.
研究的目的:
- 研究突触蛋白在突触囊泡池组织中的作用.
- 为了确定神经递质释放中的突触素相关囊泡的功能意义.
主要方法:
- 使用显微镜在释放部位的突触囊泡池的表征.
- 突触前注射突触素抗体以评估池动态.
- 在不同刺激频率下,对神经递质释放的电生理学评估.
主要成果:
- 突触囊泡聚集成一个含有突触素的远端池和一个没有突触素的近端池.
- 突触素抗体选择性地消除了远端囊泡池.
- 远端池的耗尽显著损害了高频 (18-20 Hz) 神经递质释放,但没有低频 (0.2 Hz) 释放.
结论:
- 与突触素相关的远端囊泡池对于在高频神经活动期间维持神经递质释放至关重要.
- 突触素在维持持续神经传递所需的容易释放的囊泡池中发挥着至关重要的作用.
相关概念视频
Synaptic Signaling
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
The Synapse
Neurons communicate with one another by passing on their electrical signals to other neurons. A synapse is the location where two neurons meet to exchange signals. At the synapse, the neuron that sends the signal is called the presynaptic cell, while the neuron that receives the message is called the postsynaptic cell. Note that most neurons can be both presynaptic and postsynaptic, as they both transmit and receive information.
Fusion of Secretory Vesicles with the Plasma Membrane
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
Synaptic Signaling
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...

