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Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
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用边界元素相关因子BEAF-32可视化染色体域
K Zhao1, C M Hart, U K Laemmli
1Department of Biochemistry, University of Geneva, Switzerland.
Cell
|June 16, 1995
概括
研究人员确定了BEAF-32,这是一种新型蛋白质,它与Drosophila中的scs的边界元素结合. 这种蛋白质对染色体域组织和增强剂阻断活性至关重要,这表明它对染色质结构有作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 染色体生物学 染色体生物学
背景情况:
- 在Drosophila中的hsp70位点包含了scs的边界元素,这是一个对域绝缘至关重要的调节区域.
- 了解结合并调节边界元素功能的因素是理解基因组组织的关键.
研究的目的:
- 为了识别和描述与scs的边界元素结合的蛋白质.
- 研究这些蛋白质在增强剂阻断和染色体域结构中的作用.
主要方法:
- 来自Drosophila的蛋白质净化.
- cDNA克隆和测序. 克隆和测序.
- 在体内结合试验.体内结合试验.
- 聚烯染色体的免疫染色.
- 增强剂阻断测试.
主要成果:
- 净化了与scs'元素结合的两个蛋白质;其中一个被确定为BEAF-32 (边界元素关联因子32kDa).
- BEAF-32与scs结合,但在体内并没有结合scs.
- 免疫染揭示了BEAF-32的局部化到聚烯染色体上的跨带和膨胀边界.
- BEAF-32的Palindromic结合部位与边界功能有关,尽管在短暂转染细胞中增强剂阻断试验是负的,这表明染色质参与.
结论:
- BEAF-32是一种新型蛋白质,它与scs的边界元素结合,并局部化到跨带和膨胀边界.
- scs的结合部位在边界功能中发挥作用,可能由BEAF-32.2调解.
- 染色体和/或核结构可能参与scs边界元素的增强剂阻断活性.
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