在反复发生自发流产的妇女中,T-辅助1型对热囊细胞的免疫力
J A Hill1, K Polgar, D J Anderson
1Fearing Research Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass 02115, USA.
JAMA
|June 28, 1995
概括
患有不明原因的复发性流产 (URA) 的女性表现出T-辅助1 (TH1) 免疫力对热囊细胞,可能导致生殖失败. 相反,TH2型免疫可以支持成功怀孕.
科学领域:
- 免疫学 免疫学 免疫学
- 生殖医学 生殖医学
- 细胞生物学 细胞生物学
背景情况:
- 无法解释的复发性流产 (URA) 影响了许多女性,其免疫学基础尚未完全理解.
- 免疫反应,特别是T辅助细胞分化在怀孕成功或失败中的作用是研究的一个关键领域.
研究的目的:
- 为了调查假设,外周血液单核细胞在妇女URA产生T-辅助1 (TH1) 类型的细胞因子作为对热囊细胞抗原的反应.
- 与生殖正常对照相比,以区分URA妇女的免疫反应.
主要方法:
- 一项队列研究涉及244名URA妇女,13名生殖正常的对照妇女和10名男性.
- 周围血液单核细胞被用 trofhoblast 抗原刺激.
- 用ELISA评估了超活体对小鼠胚胎的胚胎毒性作用,并分析了细胞因子 (IFN-gamma,IL-2,TNF-beta,IL-4,IL-10,TNF-alpha) 的样本.
主要成果:
- 在244名URA患者中有160名检测到胚胎毒性活性,与干扰素玛 (IFN-玛) 的存在有显著关联.
- 在URA患者表现出TH1型细胞因子 (TNF-alpha,TNF-beta) 作为对热囊细胞抗原的反应.
- 生殖正常的对照显示出最小的胚胎毒性和主要的TH2型细胞因子 (IL-10,IL-4),表明有保护性免疫反应.
结论:
- 针对热囊细胞抗原的TH1型免疫与URA有关,可能导致生殖功能衰竭.
- TH2型免疫似乎是对热囊细胞的自然和潜在有益的反应,支持成功怀孕.
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