神经蛋白集中在神经肌肉突触处,并激活ACH受体基因表达
S A Jo1, X Zhu, M A Marchionni
1Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115.
Nature
|January 12, 1995
概括
神经激活肌肉细胞中的乙胆受体 (AChR) 基因表达,这表明它是突触部位的ACHR基因转录的信号. 这种信号涉及骨肌肉中的erbB2和erbB3受体.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 两个途径调节骨肌肉突触中的乙胆受体 (AChR) 局部化.
- 阿格林信号 AChR 重新分配,而另一个途径的信号 AChR 基因转录仍然未知.
研究的目的:
- 调查神经调节素是否是激活突触核中AChR基因表达的信号.
- 为了阐明神经调节蛋白在骨肌肉中的信号传递机制.
主要方法:
- 评估了Neuregulin对C2肌肉细胞中AChR基因表达的影响.
- 在ACHR三角形子单元基因中确定了神经调节蛋白反应元件.
- 用抗体将神经蛋白定位在突触部位.
- 在C2肌肉细胞中分析了erbB2,erbB3和erbB4mRNA的表达.
- 测量了neregulin诱导的erbB2和erbB3的铁酸化.
主要成果:
- 神经激活了C2肌肉细胞中的ACHR基因表达.
- 神经调节蛋白反应元素位于负责突触特异性表达的181个基对内.
- 神经蛋白集中在突触部位,并独立于神经和肌肉持续存在.
- C2肌肉细胞表达了erbB2和erbB3,神经素刺激了它们的氨酸酸化.
结论:
- 神经素是激活信号的可能候选者 在突触部位激活 AChR 基因转录.
- 骨肌中的神经调节信号可能由erbB2/erbB3受体复合体介导.
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