概括
在一些瘤和转化细胞中,C组腺病毒表达一种特定的DNA结合蛋白. 这种病毒蛋白是病毒的蛋白质.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 瘤发生的发生因子.
背景情况:
- C组腺病毒编码了一个72,000达尔顿的单链特异性DNA结合蛋白.
- 这种蛋白质是在生产性病毒感染期间早期合成的.
研究的目的:
- 为了研究这种DNA结合蛋白在腺病毒诱导的瘤和转化细胞中的表达.
- 为了确定特定病毒DNA片段的存在与蛋白质表达是否相关.
主要方法:
- 利用来自腺病毒瘤的仓鼠的抗血清来检测对DNA结合蛋白的抗体.
- 分析了五种2型腺病毒转换的老鼠细胞系,以检测单链特异性DNA结合蛋白的存在.
- 与腺病毒基因组的Eco R-I-B限制酶片段的存在相关联的蛋白质表达.
主要成果:
- 从瘤携带的仓鼠中测试的两种抗血清之一与DNA结合蛋白反应.
- 在5种2型腺病毒转换的老鼠细胞系中,有2种表达出可检测的病毒DNA结合蛋白水平.
- 在含有Eco R-I-B片段的转化细胞中观察到DNA结合蛋白的表达,但在缺乏它的细胞中没有.
结论:
- C组腺病毒单链特异性DNA结合蛋白在腺病毒瘤和转化细胞系的子集中表达.
- 这种蛋白质的结构基因位于Eco R-I-B DNA片段内.
- 在转化细胞中存在Eco R-I-B片段,导致病毒DNA结合蛋白的表达.
更多相关视频
10:22Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Published on: November 12, 2015
9.3K
08:14Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
Published on: October 26, 2018
9.0K
相关概念视频
00:42Cross-reactivity
Overview
01:29Coronavirus
Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...
01:25Hepatitis
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...