树突细胞的原始细胞被一种有条件的v-Rel雌激素受体融合蛋白v-RelERER转化
G Boehmelt1, J Madruga, P Dörfler
1Institute of Molecular Pathology, Vienna, Austria.
Cell
|January 27, 1995
概括
一种有条件的v-Rel雌激素受体融合蛋白 (v-RelER) 转化骨髓细胞. 无活化后,这些细胞分化为树突细胞或中性粒细胞,这表明v-RelER的目标是共同的祖先.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 该v-Rel蛋白是一种已知的瘤基因,参与细胞转化.
- 条件融合蛋白提供了以时间和剂量依赖的方式研究基因功能的工具.
研究的目的:
- 研究由有条件的v-Rel雌激素受体融合蛋白 (v-RelER) 转换的细胞的分化潜力.
- 为了确定v-RelER和真实v-Rel.目标的原始细胞类型.
主要方法:
- 使用v-RelER.使用骨髓细胞的条件转化.
- 使用雌激素抗剂无活化v-RelER活性.
- 分化细胞的形态和功能分析.
- 与v-rel转化细胞进行比较.
主要成果:
- 转化为v-RelER的细胞最初表现出B淋巴体的特征.
- 无效化v-RelER导致分化成抗原呈现树突细胞.
- 在不同的条件下,v-relER细胞分化为多态核中性粒细胞.
结论:
- 有条件的v-RelER在非激活时诱导分化成不同的细胞类型.
- v-RelER,以及可能的v-Rel,转化一个共同的原始细胞,可以产生中性粒细胞和树突细胞.
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