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蛋白激酶A在刺信号传导和Drosophila影像盘发育中的功能

W Li¹, J T Ohlmeyer, M E Lane

¹Department of Biological Sciences, Columbia University, New York, New York 10027.

|February 24, 1995

概括

图像盘中蛋白激酶A (PKA) 活性降低触发基因表达,导致模式重复. 这表明PKA对于刺信号如何控制发育至关重要.

科学领域:

发育生物学是发展生物学.
分子遗传学分子遗传学
细胞信号传递细胞信号传递

背景情况:

刺 (hh) 信号对于图像盘发育和图案形成至关重要.
蛋白激酶A (PKA) 是细胞信号转导通路中的一个关键酶.

研究的目的:

调查PKA活性在调节Drosophila影像盘中的基因表达和模式形成中的作用.
为了确定PKA在这个过程中是否在刺信号的上游或下游作用.

主要方法:

在Drosophila影像盘的特定区域中对PKA活性进行基因操纵.
对十头 (dpp),无翼 (wg) 和补丁 (ptc) 的基因表达量的定量分析.
观察和分析成人的翅膀和腿形态,以发现模式重复.

主要成果:

减少PKA活性诱导dpp,wg和ptc转录独立于hh.
这些变化导致了类似于宫外HH表达引起的模式重复.
PKA抑制模仿hh活动促进盘生长,而PKA过度表达抵消了hh诱导的ptc表达.

结论:

PKA是形象性磁盘中hh信号通路的组成部分.
PKA调节了dpp,wg和ptc等关键模式分子的表达.
PKA活动影响细胞自主基因表达和非自主模式形成.

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