2.5A分辨率的配对域-DNA复合体的晶体结构揭示了Pax发育突变的结构基础
1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Cell
|February 24, 1995
概括
多虫配对的 (prd) 蛋白质
科学领域:
- 结构生物学 结构生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 多虫配对 (prd) 蛋白对胚胎发育至关重要.
- 了解它的DNA结合机制是解读基因调节的关键.
研究的目的:
- 为了阐明Drosophila配对 (prd) 蛋白质DNA结合的结构基础.
- 研究其N端和C端子域在DNA相互作用中的作用.
主要方法:
- 使用X射线晶体学来确定一个共晶体的2.5 Å分辨率结构.
- 共同晶体包含了Drosophila配对 (prd) 蛋白质的配对域和一个15bp的DNA位点.
主要成果:
- 配对域由独立的N-终端和C-终端子域组成.
- N终端域通过一种新的β转动动机和螺旋-转-螺旋单元进行广泛的DNA接触.
- C终端域对于DNA结合并非必不可少,并且不会与DNA接触.
结论:
- N-终端子域对于DNA结合至关重要,利用独特的结构特征.
- 在N-终端子域中识别的蛋白质-DNA接口与哺乳动物Pax基因中已知的突变部位相关.
- 这些发现提供了对配对盒蛋白的结构功能关系的见解.
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