在bcr-null突变体中增加了中性粒细胞呼吸突发
J W Voncken1, H van Schaick, V Kaartinen
1Department of Pathology, Childrens Hospital of Los Angeles 90027.
Cell
|March 10, 1995
概括
Bcr蛋白调节中性粒细胞功能和超氧化物产生,影响败血症冲击和组织损伤. 这一发现将Bcr与Bcr联系起来.
科学领域:
- 血液学 血液学 血液学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 费城 (Ph) 阳性白血病的特征是BCR-ABL融合基因.
- BCR-ABL蛋白与白血病的发展有关.
- 中性粒细胞在炎症反应和组织损伤中发挥着关键作用.
研究的目的:
- 调查Bcr在中性粒细胞功能中的体内作用.
- 为了确定BCR缺乏对败血症休克和组织损伤的影响.
- 探索Bcr与NADPH氧化酶系统之间的联系.
主要方法:
- 使用的bcr-null突变小鼠暴露于格拉姆阴性内毒素.
- 评估中性粒细胞激活,反应性氧代谢物生产和p21rac2膜转位.
- 比较Bcr缺乏 (bcr-/-) 和野生型 (bcr+/+) 中性粒细胞之间的反应.
主要成果:
- 缺乏Bcr的小鼠表现出对感染性休克和组织损伤的敏感性增加.
- 来自bcr (-/-) 小鼠的活性中性粒细胞显示出明显更高的活性氧代谢物产量.
- 与BCR (+/+) 中性粒细胞相比,活性BCR (-/-) 中性粒细胞的p21rac2膜转位增加了3倍.
结论:
- 在生物体内,BCR在调节白细胞NADPH氧化酶的Rac介导超氧化物产生方面发挥着至关重要的作用.
- 由于BCR缺乏的中性粒细胞过活化会导致严重的败血症休克和组织损伤.
- 这些发现表明Bcr功能与Ph阳性白血病的发病之间存在潜在的联系.
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