在Drosophila胚胎的后部模式中,需要将pumilio与母驼的mRNA结合起来
1Howard Hughes Medical Institute, Department of Genetics, Duke University Medical Center, Durham, North Carolina 27710.
Cell
|March 10, 1995
概括
包括Pumilio (pum) 在内的两种蛋白质,与驼背的mRNA中的纳米响应元素 (NREs) 结合. 这种结合对于Drosophila胚胎的后部模式至关重要,这表明pump招募纳米来调节翻译.
科学领域:
- 发育生物学是发展生物学.
- 基因调节 基因调节
- 分子机制的分子机制
背景情况:
- 在Drosophila胚胎的后部模式依赖于纳米 (nos) 蛋白活性.
- 纳米抑制背 (hb) 基因翻译通过特定的mRNA网站称为纳米响应元素 (NREs).
- 纳米和NREs之间的直接相互作用以前没有被描述.
研究的目的:
- 为了识别与NREs结合的蛋白质.
- 为了研究NRE结合蛋白的体内相关性.
- 为了阐明纳米介导转化抑制的分子机制.
主要方法:
- 使用胚胎提取物进行体外结合试验,以确定NRE结合因子.
- 在体内功能测试以与NRE结合与基因抑制相关联.
- 对已识别的NRE结合蛋白进行基因分析.
主要成果:
- 两种不同于纳米的新型蛋白质在体外被发现特别与NREs结合.
- 这些因素在体外与NREs的结合与它们在体内的功能相关.
- 一个已识别的NRE结合蛋白被pumilio (pum) 基因编码,这对于细分至关重要.
结论:
- Pumilio (pum) 蛋白质识别了NREs,并且可能将纳米 (nos) 招募到巴 (hb) mRNA.
- 建议 pum/nos 复合物抑制翻译机制.
- 这种机制对于在Drosophila胚胎中建立后部模式和腹部细分至关重要.
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