艾滋病毒-1抗体血清阴性与尿液阳性
H B Urnovitz1, M Clerici, G M Shearer
1Calypte Biomedical Corporation, Berkeley, CA 94710.
Lancet (London, England)
|December 11, 1993
概括
研究了血清和尿液酶免疫测试 (EIA) 之间的不一致的HIV-1测试结果. 细胞介导免疫检测可能有助于澄清假阳性或负性HIV-1抗体结果.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 诊断测试 诊断测试 诊断测试
背景情况:
- 标准的HIV-1抗体检测依赖于血清酶免疫测试 (EIA) 和西斑 (WB).
- 血清EIA,尿液EIA和WB之间可能发生不一致的结果,使诊断复杂化.
- 细胞介导免疫反应 (CMI) 为HIV-1感染提供了一种替代措施.
研究的目的:
- 评估细胞介导免疫反应测试在解决不一致的HIV-1结果中的实用性.
- 将HIV-1特定细胞介导免疫与血清和尿液中的抗体检测方法进行比较.
主要方法:
- 进行了两项研究,涉及具有不一致的HIV-1检测结果的个体.
- 参与者接受了血清EIA,尿液EIA,西部斑块和细胞介导免疫反应测试,以检测HIV-1的活性.
- 后续评估包括重复测试和细胞介导免疫反应评估.
主要成果:
- 在第一项研究中,7名患者血清EIA呈阴性,但尿液EIA和WB呈阳性,CMI反应混合.
- 在第二项研究中,5名受试者中有4名通过尿液EIA呈阳性,通过血清WB不确定的/阴性,他们通过CMI对HIV-1活性呈阳性.
- CMI测试表明,在具有不一致抗体测试结果的情况下,有可能识别真正的HIV-1感染.
结论:
- 细胞介导免疫反应测试显示,在解决不一致的HIV-1抗体测试结果方面有希望.
- 通过将细胞介导免疫与尿液抗体反应进行比较,可以提高HIV-1诊断的准确性.
- 需要进一步的研究来验证CMI作为HIV-1感染的确认测试.
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