基于地图的克隆蛋白激酶基因,使番茄具有抗病能力
G B Martin1, S H Brommonschenkel, J Chunwongse
1Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14853-1902.
番茄植物获得了对Pseudomonas syringae pv. 番茄通过Pto基因. 这种基因与蛋白质激酶相似,可能在植物防御信号通路中起作用.
科学领域:
- 植物病理学 植物病理学
- 分子遗传学 分子遗传学
- 植物与微生物的相互作用
背景情况:
- 番茄中的Pto基因提供了对Pseudomonas syringae pv.特定菌株的抵抗力. 番茄 番茄 番茄 番茄 番茄
- 携带激烈性基因avrPto的病原体种族是这种耐药性的目标.
研究的目的:
- 识别和表征负责番茄中介抗体的基因.
- 为了阐明这种植物防御反应背后的分子机制.
主要方法:
- 酵母人造染色体 (YAC) 克隆,以隔离Pto基因组区域.
- 使用YAC克隆对叶子互补DNA (cDNA) 库进行选.
- 基因分析以确认候选基因与Pto.的共同分离.
- 易受候选cDNA的番茄植物的转化.
- 分离基因的氨基酸序列分析.
主要成果:
- 确定了一种与Pto相关的基因家族,至少有六名成员在遗传上与抗性位点共同分离.
- 具有该家族cDNA的易受植物的转化赋予了病原体耐药性.
- 推断出的氨基酸序列与氨酸-氨酸蛋白质激酶具有相似性.
结论:
- 普托基因家族在番茄对抗Pseudomonas syringae pv的防御中发挥着至关重要的作用. 番茄 番茄 番茄 番茄 番茄
- 可能作为一种氨酸-氨酸蛋白激酶,参与植物抗病信号转导通路的功能.
更多相关视频
05:58Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
Published on: August 20, 2018
09:05Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
相关概念视频
Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Test Cross
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Pharmacogenomics: Identification of New Drug Targets
