FGF5作为头发生长周期的调节者:有针对性和自发突变的证据
J M Hébert1, T Rosenquist, J Götz
1Department of Anatomy and Programs in Genetics and in Developmental Biology, School of Medicine, University of California, San Francisco 94143.
Cell
|September 23, 1994
概括
纤维细胞生长因子5 (FGF5) 抑制头发的延长. 在小鼠身上进行的遗传研究显示FGF5是FGF5.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 皮肤病学 皮肤病学
背景情况:
- 纤维细胞生长因子5 (FGF5) 是一种被分泌的信号蛋白,参与各种生物过程.
- 在小鼠中发生的自发突变安哥拉 (go) 具有异常长的毛发的特征.
- FGF5在毛发生长调节中的遗传基础和功能尚未完全理解.
研究的目的:
- 调查纤维细胞生长因子5 (FGF5) 在头发生长调节中的作用.
- 为了确定对小鼠安哥拉 (GO) 突变负责的基因.
- 阐明FGF5影响头发循环的机制.
主要方法:
- 在胚胎干细胞中准基因以创建fgf5新突变小鼠.
- 对于fgf5neo和go突变的同卵性小鼠的表型分析.
- 在fgf5neo和go突变之间进行补充测试.
- 用DNA分析识别Fgf5基因中的突变.
- 在毛发生长周期期间,在小鼠毛囊中分析Fgf5的表达.
主要成果:
- 具有Fgf5 (fgf5neo) 预测无等位基因的小鼠表现出异常长的毛发.
- fgf5neo小鼠的表型与自发性安戈拉 (GO) 突变小鼠没有区别.
- fgf5neo和go突变无法补充,表明它们影响相同的基因.
- Fgf5的外因子1被删除在Go同胞体中,确认Go是Fgf5.5的突变等位基因.
- 在野生型老鼠毛囊中检测到Fgf5表达,特别是在异形VI期间的外部根盖中.
结论:
- FGF5 作为头发延长的抑制剂.
- 安戈拉 (GO) 突变是Fgf5基因的功能丧失等位基因.
- 通过控制头发的延长,FGF5在调节头发生长周期方面发挥着至关重要的作用.
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