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人类TAFII30存在于一个独特的TFIID复合体中,并且需要通过雌激素受体进行转录激活
1Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Faculté de Médecine, Strasbourg, France.
Cell
|October 7, 1994
概括
研究人员在不同的TFIID复合体内确定了一种特定的TBP相关因子 (TAF),hTAFII30. 这一发现表明,在基因转录调节方面,TFIID群体的功能不同.
科学领域:
- 分子生物学分子生物学
- 基因转录 基因转录
- 蛋白质复合体 蛋白质复合体
背景情况:
- TFIID复合体与TATA结合蛋白 (TBP) 相结合,并通过转录刺激进行介导.
- 不同的激活剂在TFIID中使用与TBP相关的不同的协同激活剂.
研究的目的:
- 为了描述一个特定的人类TBP相关因子 (TAF),hTAFII30.
- 研究hTAFII30在人类雌激素受体 (ER) 的转录调节中的功能性作用.
主要方法:
- 对hTAFII30.30的生物化学表征
- 与人类雌激素受体 (ER) 域和VP16的相互作用研究.
- 抗体介导的抑制和TFIID复合物的分离.
主要成果:
- hTAFII30被确定为与TFIID复合物的子集相关的TAF.
- hTAFII30 专门与 ER AF-2 激活域相互作用,而不是 ER AF-1 或 VP16.
- 对hTAFII30的抗体选择性抑制了ER AF-2介导的转录和分离的TFIID群体.
结论:
- 存在功能上不同的TFIID群体,共享共同的TAFII,但在特定的TAFII上有所不同.
- hTAFII30是TFIID亚群的一个组成部分,参与ER AF-2-介导的转录激活.
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