在旋转标记的背后形形素的光周期期间检测结构变化的时间解析
H J Steinhoff1, R Mollaaghababa, C Altenbach
1Institut für Biophysik, Ruhr-Universität Bochum, Germany.
Science (New York, N.Y.)
|October 7, 1994
概括
旋转标签揭示了其光循环期间的背后小素的运动. 在残留物101的特定光谱变化与质子化事件和中间状态相关,将结构与功能联系起来.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 摄影生物学 摄影生物学
背景情况:
- 细菌原是一种以光驱动的质子,对能量传导至关重要.
- 了解其光循环机制需要将结构动态与功能中间体相关联.
研究的目的:
- 为了研究其光循环过程中巴克特里奥霍多普辛的局部形状变化.
- 将光学观察到的中间体与特定的分子运动联系起来.
主要方法:
- 位点定向的突变发生,在特定位置引入囊残留物 (72, 101, 105).
- 半氨酸残留物的旋转标签.
- 电子偏磁共振 (EPR) 谱学用于监测光周期期间的光谱变化.
主要成果:
- 在残留物72和105的旋转标签的EPR光谱在光循环期间是时间独立的.
- 在残留物101的旋转标签的EPR频谱呈现出时间依赖的变化.
- 这些光谱变化与M中间体的衰变和基本状态的恢复相关.
结论:
- 运动,可能在C-D或E-F螺旋间循环中,发生在光循环期间的残留物101.
- 这种运动与阿斯巴酸盐96.6的质子变化相吻合.
- 这项研究将光学定义的光循环中间体与bacteriorhodopsin中的局部形状动态联系起来.
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