B型环林酶抑制剂p40SIC1控制了S. cerevisiae中的G1到S过渡
E Schwob1, T Böhm, M D Mendenhall
1I. M. P. Research Institute of Molecular Pathology, Vienna, Austria.
Cell
|October 21, 1994
概括
酵母细胞周期的进展需要通过G1和B型环林激活Cdc28激酶. 这项研究表明,抑制剂p40SIC1的蛋白质分解对于G1到S阶段过渡至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 酵母细胞在达到特定大小时就启动了诸如芽形成,杆体重复和DNA复制等关键过程.
- 这些事件主要通过通过G1环林 (Cln1, -2,和 -3) 激活Cdc28蛋白激酶来调节.
研究的目的:
- 调查B型 (Clb) 环林在酵母细胞周期期间DNA复制中的作用.
- 阐明控制G1到S阶段过渡的机制,重点关注Cdc28活动的调节.
主要方法:
- 对六重clb1-6突变酵母菌株的分析.
- 突变表型与缺乏泛基素结合酶的cdc34突变体的比较.
- 调查p40SIC1蛋白水平和在野生类型和突变细胞中的降解.
主要成果:
- 酵母中的DNA复制依赖于G1和B型环林的Cdc28激活.
- 一个六重的clb1-6突变在G1阶段停留,具有多个芽,类似于cdc34突变.
- 抑制剂p40SIC1通过抑制Clb结合的Cdc28阻止进入S相,并在G1/S过渡时降解.
结论:
- 在酵母中,依赖环林的激酶抑制剂p40SIC1的及时蛋白质分解对于酵母G1到S相过渡至关重要.
- B型环林在激活Cdc28进行DNA复制方面发挥着至关重要的作用.
- 抑制剂降解的调节是控制细胞循环进展的关键机制.
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...