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Updated: Jun 19, 2026
08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
无毛蛋白的抑制剂参与了切口受体信号传递
M E Fortini1, S Artavanis-Tsakonas
1Howard Hughes Medical Institute, Department of Biology, Yale University, New Haven, Connecticut 06536.
Cell
|October 21, 1994
概括
鉴定了的信号调节器,无毛的抑制剂 (Su(H) 的功能增益突变. 痕信号控制Su(H) 蛋白质定位,影响Drosophila发育中的核事件.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 痕信号对于细胞在发育过程中的命运决定至关重要.
- 无毛抑制剂 (Su(H)) 是Notch通路中的一个关键的转录因子.
- 了解Su (H) 调节提供了对发展过程的洞察力.
研究的目的:
- 为了确定影响Drosophila眼睛发育中的Notch信号的突变.
- 为了研究Su(H) 蛋白活性的调节机制.
- 为了阐明Notch和Su(H) 在细胞局部化中的关系.
主要方法:
- 在Drosophila melanogaster中进行遗传查,以分离功能获取的等位基因.
- 对异位基因特异性基因相互作用与Notch路径组件的分析.
- 细胞培养实验用于研究蛋白质局部化,使用联合表达和连接体结合试验.
- 酵母相互作用陷试验以确认蛋白质与蛋白质之间的相互作用.
主要成果:
- 单独的新型功能增益等位基因的无毛抑制剂 (Su(H)).
- 证明了Su(H),Notch,Delta,deltex和大脑之间的等位基特异性相互作用.
- 表明Notch信号调节了细胞质和细胞核之间的Su (H) 蛋白位址.
- 确定了细胞内Notch cdc10/ankyrin重复为关键的Su(H) 细胞质保留.
结论:
- 痕信号直接控制了Su(H) DNA结合蛋白的细胞定位和活性.
- 这种机制突显了细胞表面受体激活如何影响核转录.
- 这些发现为Notch介导的发育过程中基因表达的调节提供了分子基础.
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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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