一种异质二元卷环蛋白,在试验室中需要进行线粒染色体凝聚
1Department of Pharmacology, University of California, San Francisco 94143-0450.
Cell
|November 4, 1994
概括
一个新发现的染色体蛋白质复合体,XCAP-C/E,对于Xenopus蛋提取物中的线粒染色体凝结至关重要. 这种蛋白质对于细胞分裂期间染色体的组装和结构完整性至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 线粒染色体凝聚是准确细胞分裂的一个基本过程.
- 驱动染色体凝聚的分子机械仍然不完全理解.
研究的目的:
- 为了识别和描述参与线粒染色体凝聚的新型蛋白质.
- 阐明已识别的蛋白质复合物的功能和结构性质.
主要方法:
- 使用了Xenopus蛋提取物用于染色体组装的体外研究.
- 采用抗体阻断实验来评估蛋白质功能.
- 演了氨基酸序列并分析了保存的蛋白质图案.
主要成果:
- 鉴定了一种异构蛋白质复合体,XCAP-C/E,对染色体凝聚至关重要.
- 证明XCAP-C/E局部化到染色质,并在组装的染色体中形成内部结构.
- 显示的XCAP-C对于线粒体染色体结构的组装和维护都是必要的.
结论:
- XCAP-C/E复合体是线粒染色体凝聚的关键调节者.
- 鉴定出的蛋白质复合体具有保存的结构动图,表明它在物种之间具有保存的功能.
- 对这种蛋白质家族的进一步研究可能会揭示细胞分裂中的更广泛的作用.
相关概念视频
Cohesins
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Condensins
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
Attachment of Sister Chromatids
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules. Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
Cohesins
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Condensins
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...


