Myb DNA结合域的特定DNA复合物的溶液结构具有合作性识别螺旋体
K Ogata1, S Morikawa, H Nakamura
1Graduate School of Integrated Science, Yokohama City University, Japan.
Cell
|November 18, 1994
概括
Myb DNA结合域 (R2R3) 结构揭示了两个识别螺旋如何结合DNA. 这种安排通过关键氨基酸相互作用特别识别了AACNG序列.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- Myb蛋白家族在基因调节中起着至关重要的作用.
- 在myb蛋白中,包含一个DNA结合域,其中有三个串联重复 (R1,R2,R3).
研究的目的:
- 确定与DNA复合中的Myb最小DNA结合域 (R2R3) 的溶液结构.
- 阐明负责特定DNA序列识别的分子相互作用.
主要方法:
- 采用了异核多维核磁共振 (NMR) 光谱学.
- 这项研究的重点是R2R3DNA结合域复杂化与特定的DNA序列.
主要成果:
- R2R3域采用了一个结构,其中R2和R3螺旋在DNA主要槽中包装.
- 来自R2和R3的两个识别螺旋直径直接相互作用,以合作方式绑定AACNG DNA序列.
- 在AACNG中,特定的基对被氨基酸残留Asn-183,Lys-182 (R3) 和Lys-128 (R2) 识别出来.
- 在完整的R1R2R3域研究中,R1重复没有显示任何特定的DNA相互作用.
结论:
- R2和R3识别螺旋的紧密包装是Myb DNA结合的关键特征.
- 这种结构安排促进了对AACNG序列的合作和特定绑定.
- 在R1重复似乎不会有助于特定的DNA序列识别.
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