皮诺普辛是一种松腺光感受分子
T Okano1, T Yoshizawa, Y Fukada
1Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo, Japan.
Nature
|November 3, 1994
概括
研究人员在松细胞中发现了一种新的感光分子 - - 皮诺普辛. 这一发现揭示了鸟类生物钟和黑激素生产的分子机制.
科学领域:
- * 分子生物学 * 分子生物学
- * * 时间生物学
- * 视觉科学 视觉科学
背景情况:
- * 鸟类松细胞通过光信号调节昼夜节律和黑激素的产生.
- *桃体光传导途径对于了解生物钟至关重要.
- * 视网膜杆细胞和松细胞在光传导中存在相似之处.
研究的目的:
- * 为了识别鸟类松细胞中的光感受分子.
- *阐明鸟类生物钟的分子机制.
- * 为了描述克隆的桃体光受体.
主要方法:
- * 克隆编码光感受分子的松腺cDNA.
- * 互补DNA转移到培养细胞中.
- * 用11-cis-retinal. reconstitution 溶解出来的蛋白质.
主要成果:
- * 一种名为pinopsin的新型opsin是从的松果cDNA中克隆出来的.
- * 松腺素与脊椎动物视网膜素共享43-48%的氨基酸特征.
- *表达的皮诺辛与11-cis-retinal复制形成一个蓝色敏感色素 (λmax ≈ 470 nm).
- *编码pinopsin的基因在松果腺特异地表达.
结论:
- * 皮诺普辛作为鸟类松果腺中的主要光传感器.
- * 这一发现揭示了鸟类昼夜时钟的关键分子组成部分.
- * 该研究提供了pinopsin在光传导中的作用的功能证据.
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