刺蛋白生物生成中的自动蛋白解
J J Lee1, S C Ekker, D P von Kessler
1Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21205.
Science (New York, N.Y.)
|December 2, 1994
概括
刺 (hh) 基因通过自身蛋白质分裂产生两种蛋白质形式,这对胚胎发育至关重要. 缺陷的裂解会影响HH蛋白信号和模式,影响胚胎发育.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 刺 (hh) 基因家族编码细胞外信号蛋白,对各种生物体的胚胎模式至关重要.
- 了解HH蛋白加工的分子机制是解读其在发育中的作用的关键.
研究的目的:
- 研究Drosophila hh基因产品的加工过程.
- 在体内确定不同HH蛋白种的功能意义.
主要方法:
- 通过体外自蛋白解析试验对蛋白质加工的分析.
- 使用突变分析评估体内前体裂变.
- 对HH蛋白变体的信号和模式活动的评估.
主要成果:
- 德洛索菲拉hh基因通过更大的前体的内部自蛋白质分裂产生两个主要的蛋白质物种.
- 降低自身蛋白解效率的突变在体内前体裂变中受损.
- 断裂障碍与HH蛋白的信号和模式活动减少相关.
- 这两种HH蛋白种表现出不同的生化特性和组织分布.
结论:
- 自体蛋白质分裂对于产生功能性HH蛋白种类至关重要.
- HH蛋白形式的独特生化特性和组织分布可能解释了远程和短程信号传输.
- 这种处理机制对于正确的胚胎模式至关重要,由刺信号通路介导.
相关概念视频
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In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
02:11Proteins: From Genes to Degradation
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Transcription is the synthesis of RNA molecules by RNA...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
02:11Proteins: From Genes to Degradation
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Transcription is the synthesis of RNA molecules by RNA...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:13The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...