在调节转录因子c-Jun压力激活通路中的SAPK/ERK激酶-1的作用
I Sánchez1, R T Hughes, B J Mayer
1Diabetes Research Laboratory, Massachusetts General Hospital East, Charlestown.
Nature
|December 22, 1994
概括
应激激活蛋白激酶 (SAPKs) 对于细胞应激反应至关重要. 这项研究确定了SAPK/ERK激酶-1 (SEK1) 作为一种新的上游激活剂,定义了从压力到基因激活的关键信号通路.
科学领域:
- 蜂信号传输是如何进行的
- 分子生物学分子生物学
- 应激反应路径 应激反应路径
背景情况:
- 压力激活蛋白激酶 (SAPKs) 是细胞对各种压力的反应的关键调节者.
- SAPKs与基激活蛋白激酶 (MAPKs) 不同,但它们具有相关的信号级联.
- c-Jun的SAPK激活与调节基因转录有关.
研究的目的:
- 为了确定参与SAPK激活的新型蛋白激酶.
- 描述一个新发现的激酶,SAPK/ERK激酶-1 (SEK1) 在压力信号传递中的作用.
- 阐明信号级联,将细胞压力与c-Jun转录因子联系起来.
主要方法:
- 一种新型蛋白激酶的识别和表征,瑞典克朗1.
- 在体外和体内测试以评估SEK1对SAPKs的激活.
- 利用一个不活跃的SEK1突变体来研究其在阻断SAPK激活中的作用.
主要成果:
- SEK1被确定为一种与MAP激酶激酶 (MEKs) 结构相关的新型蛋白激酶.
- SEK1在体外和体内都显示出SAPKs的强烈激活.
- 一种不活跃的SEK1突变物有效地阻止了细胞外刺激诱导的SAPK激活,而不会影响MAPK通路.
结论:
- SEK1是SAPK的重要上游激活器,定义了一个关键的信号通路.
- 这条通路将细胞压力信号与c-Jun转录因子的激活连接起来.
- SEK1在压力诱导的信号级联中提供了一个新的分子环节.
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