CDK激活酶和RNA聚合酶II CTD酶TFIIH/TFIIK的关系
W J Feaver1, J Q Svejstrup, N L Henry
1Department of Structural Biology, Stanford University School of Medicine, California 94305.
Cell
|December 16, 1994
概括
酵母KIN28是RNA聚合酶II转录因子IIH (TFIIH) 的一个子单元,具有蛋白激酶活性. 人类环林依赖激酶激活激酶 (CAK) 也表现出这种类似TFIIH的活性.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 酵母遗传学 酵母遗传学
背景情况:
- RNA聚合酶II转录因子IIH (TFIIH) 对于基因转录至关重要.
- p34cdc2/CDC28家族包括参与细胞循环调节的蛋白激酶.
- 之前的研究表明KIN28,环素CCL1和人类同类MO15和环素H之间的相互作用.
研究的目的:
- 为了确定KIN28作为酵母TFIIH的亚单位.
- 描述TFIIH中KIN28的蛋白激酶活性.
- 调查酵母TFIIH和人类循环素依赖激酶激活激酶 (CAK) 之间的功能关系.
主要方法:
- 序列的确定 序列的确定
- 免疫学反应性测定试验
- 铜净化技术的使用方法
- 在体内相互作用研究的研究.
主要成果:
- 鉴定出KIN28是酵母TFIIH的一种亚单元.
- KIN28是TFIIK的一部分,TFIIH子组具有对RNA聚合酶II的C终端域 (CTD) 的激酶活性.
- 人类CAK (MO15/cyclin H) 已被证明具有CTD激酶活性,类似于酵母TFIIH.
结论:
- KIN28是酵母TFIIH的关键成分,有助于其CTD激酶功能.
- 酵母KIN28和人类CAK具有功能同质性,这表明在转录调节中存在保存机制.
- 这项研究提供了对转录因子IIH的组成和功能的见解.
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