DET1,一个负调节器的光介导的发展和基因表达在阿拉比多普西斯,编码一个新型的核局部蛋白质
A Pepper1, T Delaney, T Washburn
1Plant Biology Laboratory, Salk Institute, San Diego, California 92186.
Cell
|July 15, 1994
概括
阿拉比多普西斯DET1基因产物集成光信号来控制植物的发育. DET1在细胞核中起作用,调节基因表达,影响细胞类型特定的光反应发育.
科学领域:
- 植物分子生物学 植物分子生物学
- 光信号通道的光信号通道.
- 在发育过程中基因调节.
背景情况:
- 植物使用光信号来调节生长和发育.
- DET1基因产物是光信号传导中的潜在组成部分.
- 光信号集成到开发程序中的机制尚不清楚.
研究的目的:
- 为了识别和表征阿拉比多普西斯DET1基因.
- 研究DET1在整合光信号与植物发育中的作用.
- 阐明DET1在核基因表达调节中的功能.
主要方法:
- 在Arabidopsis中DET1位点的定位克隆.
- 对具有不同程度 DET1 活性的各种 det1 突变等位基因的分析.
- 在不同的光照条件下,对det1突变体的基因表达模式的检查.
主要成果:
- DET1被确定为一种独特的核局部蛋白质.
- 具有部分DET1活性的突变者在黑暗中表现出光生长的表型.
- det1无基因突变体在时间和空间基因表达调节方面表现出严重的缺陷.
结论:
- DET1 作为一个集光信号的核蛋白.
- DET1对于光调节基因的细胞类型特定表达至关重要.
- DET1基因产物通过光调节在调节植物发育途径方面发挥着关键作用.
更多相关视频
09:31Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
Published on: April 19, 2019
9.1K
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
1.9K
相关概念视频
Negative Regulator Molecules
32.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
32.1K
Master Transcription Regulators
6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
Transcription Attenuation in Prokaryotes
14.6K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
14.6K
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Cell Signaling in Plants
4.5K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
4.5K
Repressible Operon: trp Operon
2.7K
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
2.7K
