阿拉比多普西斯COP9是一种新型信号复合体的组成部分,它调解了发育过程中的光控制
N Wei1, D A Chamovitz, X W Deng
1Department of Biology, Yale University, New Haven, Connecticut 06520-8104.
Cell
|July 15, 1994
概括
光信号被植物光受体感知,影响了发育. 这项研究确定COP9,COP8和COP11是新型光信号通路中的关键组成部分,该通路调节了Arabidopsis.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 摄影生物学 摄影生物学
背景情况:
- 环境光对于植物发育至关重要.
- 光信号被光受体感知,但下游的机制尚未完全理解.
- 阿拉比多普西斯COP9参与抑制幼苗光形生成.
研究的目的:
- 阐明COP9在光信号传导中的作用.
- 为了确定参与光调节植物发育的新型组件.
- 了解光信号如何聚合来调节幼苗光形生成.
主要方法:
- 在Arabidopsis中进行遗传分析.
- 对COP9基因进行分子克隆.
- 对COP9复合体的生物化学表征.
主要成果:
- COP9编码了一种新的197氨基酸蛋白质.
- COP9在一个大型蛋白质复合体 (> 560 kDa) 中起作用.
- COP8和COP11对于COP9的复杂形成或稳定是必不可少的.
- 光信号汇聚以调节COP9活动,影响光形生成.
结论:
- COP9,COP8和COP11确定了光介导植物发展的新信号步骤.
- 这条通路集成了来自植物染色体和蓝光受体的信号.
- 了解这种途径是解读植物生长光控制的关键.
更多相关视频
07:55Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
13.8K
10:29Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
6.2K
相关概念视频
Photoreceptors and Plant Responses to Light
22.5K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
22.5K
Biological Clocks and Seasonal Responses
36.0K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
36.0K
The Antenna Complex
6.9K
Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency can...
6.9K
Cell Signaling in Plants
4.5K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
4.5K
Calmodulin-dependent Signaling
5.0K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.0K
