相关实验视频
Updated: May 8, 2026
12:23
Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
Published on: May 26, 2010
网氨酸是胚胎脊髓中 commissural 轴突的扩散性化疗因子
T E Kennedy1, T Serafini, J R de la Torre
1Howard Hughes Medical Institute, Department of Anatomy, University of California, San Francisco 94143-0452.
Cell
|August 12, 1994
概括
网林-1,是来自地板细胞的化学转化因子,指导脊髓轴突的发展. 网林-1和网林-2都会吸引 commissural 轴突,这表明它们在神经发育中起着至关重要的作用.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 轴指导对于神经系统发育至关重要.
- 据认为,目标细胞分泌的扩散性化学转化因子会调解轴突指导.
- 脊髓底板细胞分泌着吸引脊髓 commissural轴突的因素.
研究的目的:
- 为了研究网林-1和网林-2在脊柱 commissural轴突指导中的作用.
- 为了确定网林-1和网林-2是否作为化疗因子起作用.
主要方法:
- 在发育中的脊髓中分析网林-1和网林-2RNA表达.
- 在异质细胞中表达重组网-1和网-2.
- 试验网林表达细胞在体外吸引 commissural 轴突的能力.
主要成果:
- 网林-1RNA是由地板细胞表达的.
- 网林-2RNA在腹脊髓中表达,但不是在地板上.
- 分泌的网林-1和网林-2会在远处吸引 commissural 轴突.
结论:
- 网林-1是一种由地板细胞分泌的化学转化因子.
- 网林-1和网林-2在脊髓发育过程中可能在指导 commissural 轴突中起作用.
更多相关视频
相关概念视频
01:30Neurulation
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...
01:15Neurogenesis and Regeneration of Nervous Tissue
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...